Impaired Akt phosphorylation in B-cells of patients with common variable immunodeficiency.
Clin Immunol. 2016 Sep 21;
Authors: Yazdani R, Ganjalikhani-Hakemi M, Esmaeili M, Abolhassani H, Vaeli S, Rezaei A, Sharifi Z, Azizi G, Rezaei N, Aghamohammadi A
Abstract
Common variable immunodeficiency (CVID) is a heterogeneous group of primary immunodeficiency characterized by recurrent infectious. We evaluated whether defective PI3K/Akt/FoxO pathway could influence B-cell fate. Determination of B-cell subsets in CVD patients and healthy donors (HDs) were performed using flow cytometry. We evaluated mRNA level and protein expression of PI3K, Akt and FoxO molecules using real-time PCR and flow cytometry, respectively. Moreover, phosphorylated Akt (p-Akt) expression in B-cells has also been measured by flowcytometry. We identified a significant reduction in percentage of marginal zone like B-cells, Switched and unswitched memory B-cells, total memory B-cells and plasmablasts in patients, as these reductions of had significantly negative correlation with increased apoptosis in patients. Surprisingly, we identified decreased p-AKT expression in B-cells of patients than HDs. We describe for the first time impaired p-Akt expression in B-cells of CVID patients that had a significant correlation with antibody response to vaccine and clinical manifestations.
PMID: 27664934 [PubMed – as supplied by publisher]
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